Lactose inducible fermentation in Escherichia coli for improved production of recombinant urate oxidase: Optimization by statistical experimental designs

Abstract The enzyme urate oxidase (UOX) is used as a drug for preventing and treatment of hyperuricemia. This study deals with the statistical optimization lactose inducible fermentation production soluble recombinant Aspergillus flavus UOX. 10 independent variables were investigated by Plackett–Burman design (PBD), most significant factors further optimized central composite (CCD). PBD results indicated that glycerol, yeast extract, tryptone, affected UOX activity significantly. CCD showed maximum (19.34 U/ml) biomass concentration (44.0 g/L) can be achieved under optimum conditions glycerol 0.87 g/L, extract 9.11 g/L, tryptone 10.29 g/L, K2HPO4 1.81 g/L, 12.79 g/L. When same induction strategy was tested at shake flask, 19.34 U/mL obtained, which 12.5 folds higher than IPTG protocol. Furthermore, lower total cost (€0.7 vs. €13.5) additional feature confirmed suitability method. Collectively, our experiment methodology applied suitable tool improved using inducer.